Expression of gonococcal transferrin-binding protein 1 causes Escherichia coli to bind human transferrin.
نویسندگان
چکیده
The gene for gonococcal transferrin-binding protein 1 (TBP1) was cloned behind an inducible promoter in Escherichia coli. The resultant strain was capable of binding human transferrin with the same specificity as that of the gonococcus. E. coli expressing TBP1 did not internalize transferrin-bound iron or grow on transferrin as a sole iron source.
منابع مشابه
Manganese and Iron Binding to Human Transferrin
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متن کاملManganese and Iron Binding to Human Transferrin
The characteristics of manganese and iron binding to human apotransferrin (apo-tf) have been investigated and compared in this study. Both metal ions were taken up by human apo-tf and formed complexes, with the maximum absorbances observed at 410 and 340 nm for manganese-transferrin (Mn-tf) and 465 nm for iron-transferrin (Fe-tf). Addition of manganese (1.5 µg/ml) to the reaction mixture contai...
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عنوان ژورنال:
- Journal of bacteriology
دوره 175 8 شماره
صفحات -
تاریخ انتشار 1993